Application

Research Sub CategoryRNA Metabolism & Binding ProteinsRNA Binding Protein (RBP)

Research CategoryEpigenetics & Nuclear Function

Western blot

Anti-Dicer1 Antibody, clone 5D12.2 is a Mouse Monoclonal Antibody for detection of Dicer1 also known as DCR1, Dicer, HERNA, KIAA0928 & has been validated in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

RNA interference (RNAi) is mechanism for post-transcriptional gene silencing that leads to the loss of the activity of the gene. RNAi is activated when long dsRNAs are cleaved by the enzyme Dicer1 (RNase III family of dsRNA-specific endonucleases) into 21-23 nucleotide siRNA (small interfering RNA). These siRNAs bind proteins to form the RISC (RNA-induced silencing complex) which is activated when the double stranded siRNAs are unzipped to single stranded siRNAs. Activated RISC recognizes and binds complementary sequences in mRNA. The mRNA is cleaved and the overall effect is an inhibition of the protein expression and a loss of the gene activity. Dicer1 along with the Argonaute family of proteins (Ago1 and Ago2) share the same domain PIWI and PAZ domain that provide endonuclease activity to RNA-induced silencing complexes (RISC).

Immunogen

KLH conjugated synthetic peptide corresponding to amino acids 378-385 of human Dicer1

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

feature_concentration_valuePlease refer to lot specific datasheet.

Physical form

Protein G Chromatography

Format: Purified

Quality

Routinely evaluated by western blot on lysates from HeLa nuclear extracts.

Specificity

Human Dicer1

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

Target description

220 kDa